Main Article Content
Abstract
Lactobacillus has long been known to reconstruct the microbiome of the urogenital organs of healthy people. However, changes in lactobacillus abundance are known occurring in urogenital tract infections (UTI) patients. It is also known that there are Lactobacillus species that have specific genomic characteristics and nutritional requirements that cause them to turn into pathogens. Understanding population characteristics and taxa composition that reconstruct urine microbiome is very important for the diagnosis and treatment of urogenital infections. This review provides an update information of metagenomic sequencing to identify the urinary microbiome profile and the role of lactobacilli in healthy and UTI patients.
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References
- Abernethy, M. G., Rosenfeld, A., White, J. R., Lewicky-gaupp, C., & Kenton, K. (2017). Urinary Microbiome and Cytokine Levels in Women With Interstitial Cystitis. Obstet Gynecol, 129(3), 500–506. https://doi.org/10.1097/AOG.0000000000001892
- Ackerman, A. L., & Chai, T. C. (2019). The Bladder is Not Sterile : an Update on the Urinary Microbiome. Current Bladder Dysfunction Reports. https://doi.org/https://doi.org/10.1007/s11884-019-00543-6 OVERACTIVE
- Akter, M., Jannnat, R., & Nova, T. T. (2018). Lactobacillus species As a Cause of Urinary Tract Infection. Journal of Dhaka Medical College, 27(2), 215–217. https://doi.org/10.3329/jdmc.v27i2.45837
- Aragón, I. M., Herrera-Imbroda, B., Queipo-Ortuño, M. I., Castillo, E., Del Moral, J. S. G., Gómez-Millán, J., Yucel, G., & Lara, M. F. (2018). The Urinary Tract Microbiome in Health and Disease. European Urology Focus, 4(1), 128–138. https://doi.org/10.1016/j.euf.2016.11.001
- Bajic, P., Van Kuiken, M. E., Burge, B. K., Kirshenbaum, E. J., Joyce, C. J., Wolfe, A. J., Branch, J. D., Bresler, L., & Farooq, A. V. (2018). Male bladder microbiome relates to lower urinary tract symptoms. European Urology Focus, 6(2), 1–7. https://doi.org/10.1016/j.euf.2018.08.001
- Bi, H., Tian, Y., Song, C., Li, J., Liu, T., Chen, Z., Chen, C., Huang, Y., & Zhang, Y. (2019). Urinary microbiota - a potential biomarker and therapeutic target for bladder cancer. Journal of Medical Microbiology, 68(10), 1471–1478. https://doi.org/10.1099/jmm.0.001058
- Bresler, L., Price, T. K., Hilt, E. E., Joyce, C., Fitzgerald, C. M., & Wolfe, A. J. (2019). Female lower urinary tract microbiota do not associate with IC / PBS symptoms : a case-controlled study. International Urogynecology Journal. https://doi.org/https://doi.org/10.1007/s00192-019-03942-9
- Bukin, Y. S., Galachyants, Y. P., Morozov, I. V., Bukin, S. V., Zakharenko, A. S., & Zemskaya, T. I. (2019). The effect of 16s rRNA region choice on bacterial community metabarcoding results. Scientific Data, 6, 1–14. https://doi.org/10.1038/sdata.2019.7
- Chu, C. M., & Lowder, J. L. (2018). Diagnosis and treatment of urinary tract infections across age groups. American Journal of Obstetrics and Gynecology, 1–12. https://doi.org/10.1016/j.ajog.2017.12.231
- Curtiss, N., Balachandran, A., Krska, L., Peppiatt-Wildman, C., Wildman, S., & Duckett, J. (2018). Age, menopausal status and the bladder microbiome. European Journal of Obstetrics and Gynecology and Reproductive Biology, 228, 126–129. https://doi.org/10.1016/j.ejogrb.2018.06.011
- Darbro, B. W., Petroelje, B. K., & Doern, G. V. (2009). Lactobacillus delbrueckii as the cause of urinary tract infection. Journal of Clinical Microbiology, 47(1), 275–277. https://doi.org/10.1128/JCM.01630-08
- Deurenberg, R. H., Bathoorn, E., Chlebowicz, M. A., Couto, N., Ferdous, M., García-Cobos, S., Kooistra-Smid, A. M. D., Raangs, E. C., Rosema, S., Veloo, A. C. M., Zhou, K., Friedrich, A. W., & Rossen, J. W. A. (2017). Application of next generation sequencing in clinical microbiology and infection prevention. Journal of Biotechnology, 243, 16–24. https://doi.org/10.1016/j.jbiotec.2016.12.022
- DiGiulio, D. B., Callahan, B. J., McMurdie, P. J., Costello, E. K., Lyell, D. J., Robaczewska, A., Sun, C. L., Goltsman, D. S. A., Wong, R. J., Shawa, G., Stevenson, D. K., Holmes, S. P., & Relman, D. A. (2015). Temporal and spatial variation of the human microbiota during pregnancy. Proceedings of the National Academy of Sciences of the United States of America, 112(35), 11060–11065. https://doi.org/10.1073/pnas.1502875112
- Divyaswathi, C. S., & Gourishankar, A. (2013). Lactobacillus Causing Urinary Tract Infection in a Neonate. Journal of Medical Cases, 4(10), 682–685. https://doi.org/10.4021/jmc1454w
- DuPrey, K. M., McCrea, L., Rabinowitch, B. L., & Azad, K. N. (2012). Pyelonephritis and Bacteremia from Lactobacillus delbrueckii. Case Reports in Infectious Diseases, 2012, 1–3. https://doi.org/10.1155/2012/745743
- Frimodt-Møller, N. (2019). The urine microbiome – Contamination or a novel paradigm? EBioMedicine, 44, 20–21. https://doi.org/10.1016/j.ebiom.2019.05.016
- Gajer, P., Brotman, R. M., Bai, G., Sakamoto, J., Schütte, U. M. E., Zhong, X., Koenig, S. S. K., Fu, L., Ma, Z., Zhou, X., Abdo, Z., Forney, L. J., & Ravel, J. (2012). Temporal dynamics of the human vaginal microbiota. Science Translational Medicine, 4(132). https://doi.org/10.1126/scitranslmed.3003605
- Gottschick, C., Deng, Z. L., Vital, M., Masur, C., Abels, C., Pieper, D. H., Rohde, M., Mendling, W., & Wagner-Döbler, I. (2017). Treatment of biofilms in bacterial vaginosis by an amphoteric tenside pessary-clinical study and microbiota analysis. Microbiome, 5(1), 119. https://doi.org/10.1186/s40168-017-0326-y
- Greenbaum, S., Greenbaum, G., Moran-Gilad, J., & Weintruab, A. Y. (2019). Ecological dynamics of the vaginal microbiome in relation to health and disease. American Journal of Obstetrics and Gynecology, 220(4), 324–335. https://doi.org/10.1016/j.ajog.2018.11.1089
- Hasman, H., Saputra, D., Sicheritz-Ponten, T., Lund, O., Svendsen, C. A., Frimodt-Moller, N., & Aarestrup, F. M. (2014). Rapid whole-genome sequencing for detection and characterization of microorganisms directly from clinical samples. Journal of Clinical Microbiology, 52(1), 139–146. https://doi.org/10.1128/JCM.02452-13
- Horwitz, D., McCue, T., Mapesa, A. C., Ajamic, N. J., Petrosinoc, J. F., Ramigd, R. F., & Trautner, B. W. (2016). Decreased microbiota diversity associated with urinary tract infection in a trial of bacterial interference. Physiology & Behavior, 176(1), 139–148. https://doi.org/10.1016/j.physbeh.2017.03.040
- Kim, J.-M., & Park, Y.-J. (2018). Lactobacillus and Urine Microbiome in Association with Urinary Tract Infections and Bacterial Vaginosis . Urogenital Tract Infection, 13(1), 7. https://doi.org/10.14777/uti.2018.13.1.7
- Komesu, Y. M., Richter, H. E., Carper, B., Dinwiddie, D. L., Lukacz, E. S., Siddiqui, N. Y., Sung, V. W., Zyczynski, H. M., Ridgeway, B., Rogers, R. G., Arya, L. A., Mazloomdoost, D., & Gantz, M. G. (2018). The urinary microbiome in women with mixed urinary incontinence compared to similarly aged controls. International Urogynecology Journal, 29(12), 1785–1795. https://doi.org/10.1007/s00192-018-3683-6
- Macklaim, J. M., Fernandes, A. D., Di Bella, J. M., Hammond, J. A., Reid, G., & Gloor, G. B. (2013). Comparative meta-RNA-seq of the vaginal microbiota and differential expression by Lactobacillus iners in health and dysbiosis. Microbiome, 1(1), 1–11. https://doi.org/10.1186/2049-2618-1-12
- Meriwether, K. V, Lei, Z., Singh, R., & Gaskins, J. (2019). The Vaginal and Urinary Microbiomes in Premenopausal Women With Interstitial Cystitis / Bladder Pain Syndrome as Compared to Unaffected Controls : A Pilot Cross-Sectional Study. Frontiers in Cellular and Infection Microbiology, 9(April). https://doi.org/10.3389/fcimb.2019.00092
- Moustafa, A., Li, W., Singh, H., Moncera, K. J., Torralba, M. G., Yu, Y., Manuel, O., Biggs, W., Venter, J. C., Nelson, K. E., Pieper, R., & Telenti, A. (2018). Microbial metagenome of urinary tract infection. Scientific Reports, 8(1), 1–12. https://doi.org/10.1038/s41598-018-22660-8
- Muhleisen, A. L., & Herbst-Kralovetz, M. M. (2016). Menopause and the vaginal microbiome. Maturitas, 91, 42–50. https://doi.org/10.1016/j.maturitas.2016.05.015
- Neugent, M. L., Hulyalkar, N. V., Nguyen, V. H., Zimmern, P. E., & De Nisco, N. J. (2020). Advances in understanding the human urinary microbiome and its potential role in urinary tract infection. MBio, 11(2), 1–15. https://doi.org/10.1128/mBio.00218-20
- Nickel, J. C., Stephens-Shields, A. J., Landis, J. R., Mullins, C., van Bokhoven, A., Lucia, M., Henderson, J., Sen, B., Krol, J., & Ehrlich, G. (2019). A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network. Journal of Clinical Medicine, 8(3), 415. https://doi.org/10.3390/jcm8030415
- O’Hanlon, D., Come, R., & Moench, T. (2019). Vaginal pH measured in vivo : lactobacilli determine pH and lactic acid concentration. BMC Microbiology, 19(13), 1–8.
- Oulas, A., Pavloudi, C., Polymenakou, P., Pavlopoulos, G. A., Papanikolaou, N., Kotoulas, G., Arvanitidis, C., & Iliopoulos, I. (2015). Metagenomics: Tools and insights for analyzing next-generation sequencing data derived from biodiversity studies. Bioinformatics and Biology Insights, 9, 75–88. https://doi.org/10.4137/BBI.S12462
- Petrova, M. I., Reid, G., Vaneechoutte, M., & Lebeer, S. (2017). Lactobacillus iners: Friend or Foe? Trends in Microbiology, 25(3), 182–191. https://doi.org/10.1016/j.tim.2016.11.007
- Pohl, H. G., Groah, S. L., Pérez-Losada, M., Ljungberg, I., Sprague, B. M., Chandal, N., Caldovic, L., & Hsieh, M. (2020). The urine microbiome of healthy men and women differs by urine collection method. International Neurourology Journal, 24(1), 41–51. https://doi.org/10.5213/inj.1938244.122
- Price, T. K., Hilt, E. E., Thomas-white, K., Mueller, E. R., & Wolfe, A. J. (2019). The urobiome of continent adult women : a cross-sectional study. BJOG, 1–9. https://doi.org/10.1111/1471-0528.15920
- Rocca, M. F., Aguerre, L., Cipolla, L., Martínez, C., Armitano, R., Dangiolo, G., & Prieto, M. (2018). Lactobacillus spp. invasive infections in Argentina. International Journal of Infectious Diseases, 73, 163. https://doi.org/10.1016/j.ijid.2018.04.3783
- Rossi, F., Amadoro, C., & Colavita, G. (2019). Members of the lactobacillus genus complex (LGC) as opportunistic pathogens: A review. Microorganisms, 7(5), 1–15. https://doi.org/10.3390/microorganisms7050126
- Schneeweiss, J., Koch, M., & Umek, W. (2016). The human urinary microbiome and how it relates to urogynecology. International Urogynecology Journal, 27(9), 1307–1312. https://doi.org/10.1007/s00192-016-2944-5
- Siddiqui, H., Lagesen, K., Nederbragt, A. J., Jeansson, S. L., & Jakobsen, K. S. (2012). Alterations of microbiota in urine from women with interstitial cystitis. BMC Microbiology, 12(1), 1. https://doi.org/10.1186/1471-2180-12-205
- Stapleton, A. E. (2016). The Vaginal Microbiota and Urinary Tract Infection. Urinary Tract Infections, 4(6), 79–86. https://doi.org/10.1128/9781555817404.ch5
- Thomas-White, K., Forster, S. C., Kumar, N., Van Kuiken, M., Putonti, C., Stares, M. D., Hilt, E. E., Price, T. K., Wolfe, A. J., & Lawley, T. D. (2018). Culturing of female bladder bacteria reveals an interconnected urogenital microbiota. Nature Communications, 9(1), 1–7. https://doi.org/10.1038/s41467-018-03968-5
- Ursell, L. K., Metcalf, J. L., Parfrey, L. W., & Knight, R. (2013). Defining the Human Microbiome. NIH Manuscripts, 70(Suppl 1), 1–12. https://doi.org/10.1111/j.1753-4887.2012.00493.x.Defining
- Van Houdt, R., Ma, B., Bruisten, S. M., Speksnijder, A. G. C. L., Ravel, J., & De Vries, H. J. C. (2018). Lactobacillus iners-dominated vaginal microbiota is associated with increased susceptibility to Chlamydia trachomatis infection in Dutch women: A case-control study. Sexually Transmitted Infections, 94(2), 117–123. https://doi.org/10.1136/sextrans-2017-053133
- Ventolini, G. (2015). Vaginal lactobacillus: Biofilm formation in vivo – clinical implications. International Journal of Women’s Health, 7, 243–247. https://doi.org/10.2147/IJWH.S77956
- Whiteside, S. A., Razvi, H., Dave, S., Reid, G., & Burton, J. P. (2015). The microbiome of the urinary tract - A role beyond infection. Nature Reviews Urology, 12(2), 81–90. https://doi.org/10.1038/nrurol.2014.361
- Winand, R., Bogaerts, B., Hoffman, S., Lefevre, L., Delvoye, M., Van Braekel, J., Fu, Q., Roosens, N. H. C., De Keersmaecker, S. C. J., & Vanneste, K. (2020). Targeting the 16s rRNA gene for bacterial identification in complex mixed samples: Comparative evaluation of second (illumina) and third (oxford nanopore technologies) generation sequencing technologies. International Journal of Molecular Sciences, 21(1), 1–22. https://doi.org/10.3390/ijms21010298
- Wu, P., Chen, Y., Zhao, J., Zhang, G., Chen, J., Wang, J., & Zhang, H. (2017). Urinary microbiome and psychological factors in women with Overactive bladder. Frontiers in Cellular and Infection Microbiology, 7(NOV), 1–11. https://doi.org/10.3389/fcimb.2017.00488
References
Abernethy, M. G., Rosenfeld, A., White, J. R., Lewicky-gaupp, C., & Kenton, K. (2017). Urinary Microbiome and Cytokine Levels in Women With Interstitial Cystitis. Obstet Gynecol, 129(3), 500–506. https://doi.org/10.1097/AOG.0000000000001892
Ackerman, A. L., & Chai, T. C. (2019). The Bladder is Not Sterile : an Update on the Urinary Microbiome. Current Bladder Dysfunction Reports. https://doi.org/https://doi.org/10.1007/s11884-019-00543-6 OVERACTIVE
Akter, M., Jannnat, R., & Nova, T. T. (2018). Lactobacillus species As a Cause of Urinary Tract Infection. Journal of Dhaka Medical College, 27(2), 215–217. https://doi.org/10.3329/jdmc.v27i2.45837
Aragón, I. M., Herrera-Imbroda, B., Queipo-Ortuño, M. I., Castillo, E., Del Moral, J. S. G., Gómez-Millán, J., Yucel, G., & Lara, M. F. (2018). The Urinary Tract Microbiome in Health and Disease. European Urology Focus, 4(1), 128–138. https://doi.org/10.1016/j.euf.2016.11.001
Bajic, P., Van Kuiken, M. E., Burge, B. K., Kirshenbaum, E. J., Joyce, C. J., Wolfe, A. J., Branch, J. D., Bresler, L., & Farooq, A. V. (2018). Male bladder microbiome relates to lower urinary tract symptoms. European Urology Focus, 6(2), 1–7. https://doi.org/10.1016/j.euf.2018.08.001
Bi, H., Tian, Y., Song, C., Li, J., Liu, T., Chen, Z., Chen, C., Huang, Y., & Zhang, Y. (2019). Urinary microbiota - a potential biomarker and therapeutic target for bladder cancer. Journal of Medical Microbiology, 68(10), 1471–1478. https://doi.org/10.1099/jmm.0.001058
Bresler, L., Price, T. K., Hilt, E. E., Joyce, C., Fitzgerald, C. M., & Wolfe, A. J. (2019). Female lower urinary tract microbiota do not associate with IC / PBS symptoms : a case-controlled study. International Urogynecology Journal. https://doi.org/https://doi.org/10.1007/s00192-019-03942-9
Bukin, Y. S., Galachyants, Y. P., Morozov, I. V., Bukin, S. V., Zakharenko, A. S., & Zemskaya, T. I. (2019). The effect of 16s rRNA region choice on bacterial community metabarcoding results. Scientific Data, 6, 1–14. https://doi.org/10.1038/sdata.2019.7
Chu, C. M., & Lowder, J. L. (2018). Diagnosis and treatment of urinary tract infections across age groups. American Journal of Obstetrics and Gynecology, 1–12. https://doi.org/10.1016/j.ajog.2017.12.231
Curtiss, N., Balachandran, A., Krska, L., Peppiatt-Wildman, C., Wildman, S., & Duckett, J. (2018). Age, menopausal status and the bladder microbiome. European Journal of Obstetrics and Gynecology and Reproductive Biology, 228, 126–129. https://doi.org/10.1016/j.ejogrb.2018.06.011
Darbro, B. W., Petroelje, B. K., & Doern, G. V. (2009). Lactobacillus delbrueckii as the cause of urinary tract infection. Journal of Clinical Microbiology, 47(1), 275–277. https://doi.org/10.1128/JCM.01630-08
Deurenberg, R. H., Bathoorn, E., Chlebowicz, M. A., Couto, N., Ferdous, M., García-Cobos, S., Kooistra-Smid, A. M. D., Raangs, E. C., Rosema, S., Veloo, A. C. M., Zhou, K., Friedrich, A. W., & Rossen, J. W. A. (2017). Application of next generation sequencing in clinical microbiology and infection prevention. Journal of Biotechnology, 243, 16–24. https://doi.org/10.1016/j.jbiotec.2016.12.022
DiGiulio, D. B., Callahan, B. J., McMurdie, P. J., Costello, E. K., Lyell, D. J., Robaczewska, A., Sun, C. L., Goltsman, D. S. A., Wong, R. J., Shawa, G., Stevenson, D. K., Holmes, S. P., & Relman, D. A. (2015). Temporal and spatial variation of the human microbiota during pregnancy. Proceedings of the National Academy of Sciences of the United States of America, 112(35), 11060–11065. https://doi.org/10.1073/pnas.1502875112
Divyaswathi, C. S., & Gourishankar, A. (2013). Lactobacillus Causing Urinary Tract Infection in a Neonate. Journal of Medical Cases, 4(10), 682–685. https://doi.org/10.4021/jmc1454w
DuPrey, K. M., McCrea, L., Rabinowitch, B. L., & Azad, K. N. (2012). Pyelonephritis and Bacteremia from Lactobacillus delbrueckii. Case Reports in Infectious Diseases, 2012, 1–3. https://doi.org/10.1155/2012/745743
Frimodt-Møller, N. (2019). The urine microbiome – Contamination or a novel paradigm? EBioMedicine, 44, 20–21. https://doi.org/10.1016/j.ebiom.2019.05.016
Gajer, P., Brotman, R. M., Bai, G., Sakamoto, J., Schütte, U. M. E., Zhong, X., Koenig, S. S. K., Fu, L., Ma, Z., Zhou, X., Abdo, Z., Forney, L. J., & Ravel, J. (2012). Temporal dynamics of the human vaginal microbiota. Science Translational Medicine, 4(132). https://doi.org/10.1126/scitranslmed.3003605
Gottschick, C., Deng, Z. L., Vital, M., Masur, C., Abels, C., Pieper, D. H., Rohde, M., Mendling, W., & Wagner-Döbler, I. (2017). Treatment of biofilms in bacterial vaginosis by an amphoteric tenside pessary-clinical study and microbiota analysis. Microbiome, 5(1), 119. https://doi.org/10.1186/s40168-017-0326-y
Greenbaum, S., Greenbaum, G., Moran-Gilad, J., & Weintruab, A. Y. (2019). Ecological dynamics of the vaginal microbiome in relation to health and disease. American Journal of Obstetrics and Gynecology, 220(4), 324–335. https://doi.org/10.1016/j.ajog.2018.11.1089
Hasman, H., Saputra, D., Sicheritz-Ponten, T., Lund, O., Svendsen, C. A., Frimodt-Moller, N., & Aarestrup, F. M. (2014). Rapid whole-genome sequencing for detection and characterization of microorganisms directly from clinical samples. Journal of Clinical Microbiology, 52(1), 139–146. https://doi.org/10.1128/JCM.02452-13
Horwitz, D., McCue, T., Mapesa, A. C., Ajamic, N. J., Petrosinoc, J. F., Ramigd, R. F., & Trautner, B. W. (2016). Decreased microbiota diversity associated with urinary tract infection in a trial of bacterial interference. Physiology & Behavior, 176(1), 139–148. https://doi.org/10.1016/j.physbeh.2017.03.040
Kim, J.-M., & Park, Y.-J. (2018). Lactobacillus and Urine Microbiome in Association with Urinary Tract Infections and Bacterial Vaginosis . Urogenital Tract Infection, 13(1), 7. https://doi.org/10.14777/uti.2018.13.1.7
Komesu, Y. M., Richter, H. E., Carper, B., Dinwiddie, D. L., Lukacz, E. S., Siddiqui, N. Y., Sung, V. W., Zyczynski, H. M., Ridgeway, B., Rogers, R. G., Arya, L. A., Mazloomdoost, D., & Gantz, M. G. (2018). The urinary microbiome in women with mixed urinary incontinence compared to similarly aged controls. International Urogynecology Journal, 29(12), 1785–1795. https://doi.org/10.1007/s00192-018-3683-6
Macklaim, J. M., Fernandes, A. D., Di Bella, J. M., Hammond, J. A., Reid, G., & Gloor, G. B. (2013). Comparative meta-RNA-seq of the vaginal microbiota and differential expression by Lactobacillus iners in health and dysbiosis. Microbiome, 1(1), 1–11. https://doi.org/10.1186/2049-2618-1-12
Meriwether, K. V, Lei, Z., Singh, R., & Gaskins, J. (2019). The Vaginal and Urinary Microbiomes in Premenopausal Women With Interstitial Cystitis / Bladder Pain Syndrome as Compared to Unaffected Controls : A Pilot Cross-Sectional Study. Frontiers in Cellular and Infection Microbiology, 9(April). https://doi.org/10.3389/fcimb.2019.00092
Moustafa, A., Li, W., Singh, H., Moncera, K. J., Torralba, M. G., Yu, Y., Manuel, O., Biggs, W., Venter, J. C., Nelson, K. E., Pieper, R., & Telenti, A. (2018). Microbial metagenome of urinary tract infection. Scientific Reports, 8(1), 1–12. https://doi.org/10.1038/s41598-018-22660-8
Muhleisen, A. L., & Herbst-Kralovetz, M. M. (2016). Menopause and the vaginal microbiome. Maturitas, 91, 42–50. https://doi.org/10.1016/j.maturitas.2016.05.015
Neugent, M. L., Hulyalkar, N. V., Nguyen, V. H., Zimmern, P. E., & De Nisco, N. J. (2020). Advances in understanding the human urinary microbiome and its potential role in urinary tract infection. MBio, 11(2), 1–15. https://doi.org/10.1128/mBio.00218-20
Nickel, J. C., Stephens-Shields, A. J., Landis, J. R., Mullins, C., van Bokhoven, A., Lucia, M., Henderson, J., Sen, B., Krol, J., & Ehrlich, G. (2019). A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network. Journal of Clinical Medicine, 8(3), 415. https://doi.org/10.3390/jcm8030415
O’Hanlon, D., Come, R., & Moench, T. (2019). Vaginal pH measured in vivo : lactobacilli determine pH and lactic acid concentration. BMC Microbiology, 19(13), 1–8.
Oulas, A., Pavloudi, C., Polymenakou, P., Pavlopoulos, G. A., Papanikolaou, N., Kotoulas, G., Arvanitidis, C., & Iliopoulos, I. (2015). Metagenomics: Tools and insights for analyzing next-generation sequencing data derived from biodiversity studies. Bioinformatics and Biology Insights, 9, 75–88. https://doi.org/10.4137/BBI.S12462
Petrova, M. I., Reid, G., Vaneechoutte, M., & Lebeer, S. (2017). Lactobacillus iners: Friend or Foe? Trends in Microbiology, 25(3), 182–191. https://doi.org/10.1016/j.tim.2016.11.007
Pohl, H. G., Groah, S. L., Pérez-Losada, M., Ljungberg, I., Sprague, B. M., Chandal, N., Caldovic, L., & Hsieh, M. (2020). The urine microbiome of healthy men and women differs by urine collection method. International Neurourology Journal, 24(1), 41–51. https://doi.org/10.5213/inj.1938244.122
Price, T. K., Hilt, E. E., Thomas-white, K., Mueller, E. R., & Wolfe, A. J. (2019). The urobiome of continent adult women : a cross-sectional study. BJOG, 1–9. https://doi.org/10.1111/1471-0528.15920
Rocca, M. F., Aguerre, L., Cipolla, L., Martínez, C., Armitano, R., Dangiolo, G., & Prieto, M. (2018). Lactobacillus spp. invasive infections in Argentina. International Journal of Infectious Diseases, 73, 163. https://doi.org/10.1016/j.ijid.2018.04.3783
Rossi, F., Amadoro, C., & Colavita, G. (2019). Members of the lactobacillus genus complex (LGC) as opportunistic pathogens: A review. Microorganisms, 7(5), 1–15. https://doi.org/10.3390/microorganisms7050126
Schneeweiss, J., Koch, M., & Umek, W. (2016). The human urinary microbiome and how it relates to urogynecology. International Urogynecology Journal, 27(9), 1307–1312. https://doi.org/10.1007/s00192-016-2944-5
Siddiqui, H., Lagesen, K., Nederbragt, A. J., Jeansson, S. L., & Jakobsen, K. S. (2012). Alterations of microbiota in urine from women with interstitial cystitis. BMC Microbiology, 12(1), 1. https://doi.org/10.1186/1471-2180-12-205
Stapleton, A. E. (2016). The Vaginal Microbiota and Urinary Tract Infection. Urinary Tract Infections, 4(6), 79–86. https://doi.org/10.1128/9781555817404.ch5
Thomas-White, K., Forster, S. C., Kumar, N., Van Kuiken, M., Putonti, C., Stares, M. D., Hilt, E. E., Price, T. K., Wolfe, A. J., & Lawley, T. D. (2018). Culturing of female bladder bacteria reveals an interconnected urogenital microbiota. Nature Communications, 9(1), 1–7. https://doi.org/10.1038/s41467-018-03968-5
Ursell, L. K., Metcalf, J. L., Parfrey, L. W., & Knight, R. (2013). Defining the Human Microbiome. NIH Manuscripts, 70(Suppl 1), 1–12. https://doi.org/10.1111/j.1753-4887.2012.00493.x.Defining
Van Houdt, R., Ma, B., Bruisten, S. M., Speksnijder, A. G. C. L., Ravel, J., & De Vries, H. J. C. (2018). Lactobacillus iners-dominated vaginal microbiota is associated with increased susceptibility to Chlamydia trachomatis infection in Dutch women: A case-control study. Sexually Transmitted Infections, 94(2), 117–123. https://doi.org/10.1136/sextrans-2017-053133
Ventolini, G. (2015). Vaginal lactobacillus: Biofilm formation in vivo – clinical implications. International Journal of Women’s Health, 7, 243–247. https://doi.org/10.2147/IJWH.S77956
Whiteside, S. A., Razvi, H., Dave, S., Reid, G., & Burton, J. P. (2015). The microbiome of the urinary tract - A role beyond infection. Nature Reviews Urology, 12(2), 81–90. https://doi.org/10.1038/nrurol.2014.361
Winand, R., Bogaerts, B., Hoffman, S., Lefevre, L., Delvoye, M., Van Braekel, J., Fu, Q., Roosens, N. H. C., De Keersmaecker, S. C. J., & Vanneste, K. (2020). Targeting the 16s rRNA gene for bacterial identification in complex mixed samples: Comparative evaluation of second (illumina) and third (oxford nanopore technologies) generation sequencing technologies. International Journal of Molecular Sciences, 21(1), 1–22. https://doi.org/10.3390/ijms21010298
Wu, P., Chen, Y., Zhao, J., Zhang, G., Chen, J., Wang, J., & Zhang, H. (2017). Urinary microbiome and psychological factors in women with Overactive bladder. Frontiers in Cellular and Infection Microbiology, 7(NOV), 1–11. https://doi.org/10.3389/fcimb.2017.00488